The dideoxy method gets its name from the critical role played by synthetic nucleotides that lack the -OH at the 3′ carbon atom (red arrow). Each new nucleotide is added to the 3′ -OH group of the last nucleotide added. The top formula shows one of them: deoxythymidine triphosphate (dTTP). The most popular method for doing this is called the dideoxy method or Sanger method (named after its inventor, Frederick Sanger, who was awarded the 1980 Nobel prize in chemistry for this achievment).ĭNA is synthesized from four deoxynucleotide triphosphates. DNA sequencing is the determination of the precise sequence of nucleotides in a sample of DNA.
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